Passive transverse mechanical properties as a function of temperature of rat skeletal muscle in vitro.

نویسندگان

  • Mark van Turnhout
  • Gerrit Peters
  • Anke Stekelenburg
  • Cees Oomens
چکیده

The objective of the current study was to determine the in vitro passive transverse mechanical properties of skeletal muscle with Dynamic Mechanical Thermal Analysis (DMTA) tests. The starting hypotheses was that the time-temperature-superposition principle could be used to expand the DMTA results to a 1 kHz frequency range. Experiments were performed with rat hind leg skeletal muscle tissue samples on a rotational rheometer using a parallel plate geometry. Because of the small size and low modulus of the samples, the standard test geometry was altered and the samples were shifted from the center to the edge of the plates. From strain sweep tests it became clear that for strains smaller than 0.003 the muscle tissue behaves linearly. In the linear region storage moduli ranged between 24 kPa (omega = 1 rad/s) and 42 kPa (omega = 100 rad/s) at T = 4 degrees C and 22 kPa and 33 kPa at 29 degrees C within the experimental frequency range. The loss modulus decreased with increasing frequency and ranged between 7 and 4 kPa at 4 degrees C and 4.5 and 3.5 kPa at 29 degrees C. Although the properties are clearly temperature dependent, a temperature shift in phase angle delta could not be detected, thus Time Temperature Superposition is not allowed for skeletal muscle in vitro.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

بررسی فعالیت حلقه های گاما دوک عضلانی و α-γ Linkage در دم موش (Rat)

Background and purpose : Muscle spindle is responsible for the control of skeletal muscle fibers function at rest and movement cycle, and is known as servo agent for voluntary movement. Function of this kinetic neceptor is completely dependent on the γ- ring activity would bring activity of la fiber and group ÏÏ spindle muscle afferent. Âlso, there is close functional of muscle relationship b...

متن کامل

Mechano-sensitivity of denervated Solus muscle of the rat L-type Ca channel mechano-sensitivity in long-term denervated Soleus muscle of the rat

Evidences showed that physical forces, as passive stretching or active contraction, may counteract various kinds of skeletal muscle atrophy due, for instance, to muscle immobilization, pathophysiology or denervation. In accord, active muscle contraction induced by functional electrical stimulation resulted helpful to reduce the muscle atrophic state in denervated man. Moreover, there are eviden...

متن کامل

Genetic Models in Applied Physiology Selected Contribution: Merosin deficiency leads to alterations in passive and active skeletal muscle mechanics

Jannapureddy, Suneal R., Nisha D. Patel, Willy Hwang, and Aladin M. Boriek. Selected Contribution: Merosin deficiency leads to alterations in passive and active skeletal muscle mechanics. J Appl Physiol 94: 2524–2533, 2003; 10.1152/japplphysiol.01078.2002.—The role of extracellular elements on the mechanical properties of skeletal muscles is unknown. Merosin is an essential extracellular matrix...

متن کامل

Passive stiffness of rat skeletal muscle undernourished during fetal development

OBJECTIVES The aim of the study was to investigate the effect of fetal undernutrition on the passive mechanical properties of skeletal muscle of weaned and young adult rats. INTRODUCTION A poor nutrition supply during fetal development affects physiological functions of the fetus. From a mechanical point of view, skeletal muscle can be also characterized by its resistance to passive stretch. ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Biorheology

دوره 42 3  شماره 

صفحات  -

تاریخ انتشار 2005